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Laboratory Procedure

I. Powder Triturations

When mixing a small amount of a drug with a large amount of another ingredient or diluent, the process of geometric dilution is used. In this method the drug present in smaller quantity is placed in the mortar with an equal amount of the other ingredient. The two materials are triturated until they are well mixed. Then, an equal amount of the diluent is again added to the mixture, and trituration is repeated until they are intimately mixed. This procedure is repeated until all the powders have been combined and thoroughly mixed.

1.

                  Mel Batost, MD            Rx# 51301
                  123 Upendown Rd.
                  Nowhere, NC 27000 
Phone: 555-1234                         DEA # AH0079411 

Name: Stan Dupp                          Date:  Today 
Address:  123 Main Street, Chapel Hill   Phone: 555-1234 
   Rx
        Activated Charcoal      25 mg 
        Lactose qs              10 g
        Mft powder 
        Sig: Mix contents in 1 oz. H2O prn nausea 
        No Refill
                                                        
   Product selection permitted      Dispense as written 

 

Show calculations for preparing a trituration and filling the prescription.

2.

                   Mel Batost, MD             Rx# 51302
                   123 Upendown Rd.
                   Nowhere, NC 27000 
Phone: 555-1234                          DEA # AH0079411

Name: Stan Dupp                          Date:  Today 
Address:  123 Main Street, Chapel Hill   Phone: 555-1234 
   Rx
        Amaranth        6 mg
        Lactose qs      36 g
        Mft powder 
        Sig: Put 1 tsp in 8 oz. H2O as directed prn 
        2 Refills
                                                      
   Product selection permitted     Dispense as written 

 

Bulk density of lactose is 1.53

Show calculations for preparing a trituration and filling the prescription.

3.

                    Mel Batost, MD          Rx# 51303
                    123 Upendown Rd.
                    Nowhere, NC 27000 
Phone: 555-1234                       DEA # AH0079411 

Name: Stan Dupp                        Date:  Today 
Address:  123 Main Street, Chapel Hill Phone: 555-1234
  Rx
      Salicylic acid          2 mg 
      Lactose qs              6 g 
      Mft powder
      Sig: Dissolve contents in pint of H2O and use as
           directed. 
      No Refill 
                                                     
   Product selection permitted    Dispense as written 

 

Show calculations for preparing a trituration and filling the prescription.

Analysis of product: Take 0.4 gram of the salicylic acid product, dilute to 10 ml with 0.1 NaOH, and measure the absorbance on the spectrophotometer at 310 nm using UV grade cuvettes. Record the absorbance.

Absorbance___________

 

II. Liquid Aliquot

Use the liquid aliquot method to prepare, label and dispense the following prescription.

                     Mel Batost, MD          Rx# 51304
                     123 Upendown Rd. 
                     Nowhere, NC 27000 
Phone: 555-1234                          DEA # AH0079411

Name: Stan Dupp                          Date:  Today 
Address:  123 Main Street, Chapel Hill   Phone: 555-1234
   Rx
        Amaranth                0.0075% 
        Aqua Dist. qs ad.       O i 
        Sig: Put 1 tsp in baby's formula prn.
        No Refill
                                                         
   Product selection permitted       Dispense as written 

 

1. Show calculations:

Amaranth _____________________g (to be weighed on Rx balance)

Distilled water q.s. ______________ml (volume of solution)

Aliquot volume _________________ml

Distilled water q.s. ______________ml (to prepare final Rx product)

2. Compounding Procedure

 

 

3. Analysis of product. Take 5 mL of your product, and add 15 mL of distilled water. Mix well. Read the absorbance on the spectrophotometer at 520 nm using standard cuvettes. Record the absorbance.

 

Absorbance________________