UV/VIS Assays
Progesterone Suppositories
Progesterone Suppositories | |
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Progesterone | 200.0 mg/supp |
Silica gel | 35.0 mg/supp |
Witepsol H-15 base | s.a. * |
Mft. 12 suppositories | |
*calculated based on the calibration of the suppository mold |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: To analyze the progesterone suppositories, two suppositories were selected from the compounded preparation. Each was dissolved in 20 mL of tetrahydrofuran, and when dissolution was complete, the mixture was hand shaken to mix well. The samples were left to stand overnight. The supernatant was read.
Standard Solutions: Standard preparations of 150–250 mg/suppository of progesterone were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 200 mg per suppository
Ketoprofen PLO Emulsion
Ketoprofen PLO Emulsion | |
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Ketoprofen | 10% w/v |
Ethoxy Diglycol | 20% v/v |
Lecithin Isopropyl Palmitate Solution | 22% v/v |
Poloxamer 407 20 % Gel | qs 100% |
Mft. 10 mL |
Link to Formulation Record:
Process of Analysis:
Sample Preparation:Ketoprofen was analyzed by dissolving a 0.5 mL sample of the PLO emulsion in 20 mL of tetrahydrofuran and reading the dissolved sample.
Standard Solutions: Standard preparations of 80% to 110% of ketoprofen were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 1.0 g/10 mL
Hydrocortisone Medication Stick
Hydrocortisone Medication Stick | |
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Hydrocortisone USP, micronized | 2.5% |
White Beeswax | 20% |
Cetyl Esters Wax | 20% |
Mineral Oil | 55% |
Acacia | 5% |
Mft. 45 gm Medication Stick |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A 250 mg sample was removed from the top of the stick, dissolved in 20 mL of tetrahydrofuran, and when dissolution was complete, the mixture was hand-shaken to mix well. The samples were left to stand overnight. One (1) mL of the supernatant was further diluted with 10 mL of tetrahydrofuran and was read.
Standard Solutions: Standard preparations of 2.0% to 3.0% of hydrocortisone medication sticks were prepared in the same manner as the student preparations, and were used as a linear standard curve to determine the student preparation percentage.
Expected Concentration of API: 2.5%
Metoprolol Tablet Triturates
Metoprolol Tartrate USP625 mg
Metoprolol Tablet Triturates | |
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Sucrose, Powder FCC | qs* |
Lactose, Hydrous NF | qs* |
Mft. 50 Tablets | |
*calculated based on the calibration of the tablet triturate mold |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A tablet was selected at random from the compounded tablets and dissolved in 20mL of distilled water and read.
Standard Solutions: Standard preparations of 10-15 mg/tablet of metoprolol were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 12.5 mg per tablet
Furosemide Tablet Triturate
Furosemide USP500 mg
Furosemide Table Triturate | |
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Sucrose, Powder FCC | qs* |
Lactose, Hydrous NF | qs* |
Mft. 50 Tablets | |
*calculated based on the calibration of the tablet triturate mold |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A tablet was selected at random and dissolved into 20 mL of 75% methanol and 25% distilled water. Samples were sonicated for approximately 20 minutes until dissolved.
Standard Solutions: Standard preparations of 5-15 mg/tablet of furosemide were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 10 mg per tablet
Co-enzyme Q10 Troches
Tartrazine (Co-enzyme Q10)2.4 g
Co-enzyme Q10 Troches | |
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Aspartame | 0.55 g |
Silica Gel | 0.24 g |
Acacia | 0.5 g |
Citric Acid Monohydrate | 0.7 g |
Flavoring Oil | 2-3 drops |
Polyethylene Glycol 1450 | qs* |
Mft. 24 troches | |
*calculated based on the calibration of the troche mold |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A troche was selected at random and dissolved in 20 mL of pH 10 buffer. The samples were left to stand overnight and read.
- pH 10 buffer:
- Solution A:
- 12.37 g boric acid
- 100 mL 1.0 N sodium hydroxide
- qs 1 L distilled water
- Solution B:
- 0.1 N sodium hydroxide
- Combine 59.6 mL of Solution A with 40.4 mL of solution B to get the pH 10 buffer used for sample preparation
- Solution A:
Standard Solutions: Standard preparations of 50-150 mg/troche of tartrazine were prepared in the same manner as the student preparations, and were used as a linear standard curve to determine the student preparation amount. Make sure the standards are left to stand overnight in order to get a linear curve.
Expected Concentration of API: 100 mg tartrazine per troche
Menthol and Phenol Troches
Menthol and Phenol Troches | |
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Phenol | 0.36 g |
Menthol | 0.24 g |
Aspartame | 0.55 g |
Silica Gel | 0.24 g |
Acacia | 0.5 g |
Citric Acid Monohydrate | 0.7 g |
Polyethylene Glycol 1450 | qs* |
Mft. 24 troches |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A troche was selected at random and dissolved in 20 mL of pH 10 buffer. A second dilution was prepared by adding 2 mL of the first dilution into 20 mL of pH 10 buffer and was then read.
- pH 10 buffer:
- Solution A:
- 12.37 g boric acid
- 100 mL 1.0 N sodium hydroxide
- qs 1 L distilled water
- Solution B:
- 0.1 N sodium hydroxide
- Combine 59.6 mL of Solution A with 40.4 mL of Solution B to get the pH 10 buffer used for sample preparation.
- Solution A:
Standard Solutions: Standard preparations of 8.3-20.8 mg/troche of methanol were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount. Make sure the standards are left to stand overnight in order to get a linear curve.
Expected Concentration of API: 15 mg of phenol per troche
Lidocaine and Dental Gel
Lidocaine and Dental Gel | |
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Lidocine USP | 2% |
Carbopol 940 NF | 2% |
Alcohol USP | 90.0% |
Distilled Water | 6.0% |
Mft. 20 g of gel |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A 0.5 g sample was dissolved in 20 mL of ethanol and left to stand overnight. Without shaking the first dilution, 0.5 mL of the first dilution was pipetted into 20 mL of ethanol and read. It is important to pre-wet the pipette tip before drawing up the 0.5 mL sample.
Standard Solutions: Standard preparations of 1.5% to 2.5% lidocaine gel were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 2% lidocaine
Salicylic Acid in Flexible Collodion
Salicylic Acid in Flexible Collodion | |
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Salicylic Acid | 16.7% (w/v) |
Lactic Acid, 88% | 16.7% (v/v) |
Flexible Collodion | qs |
Mft. 30 mL of Solution |
Link to Formulation Record:
Process of Analysis:
Sample Preparation: A 0.5 mL sample was diluted into 20 mL of methanol. A second dilution was made by adding 0.1 mL of the first dilution into 9 mL of methanol. The second dilution was left to stand overnight then read.
Standard Solutions: Standard preparations of 0% to 23.3% salicylic acid in flexible collodion were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 16.7% salicylic acid