UV/VIS Assays

Link to Formulation Record:

Process of Analysis:

Sample Preparation:Ketoprofen was analyzed by dissolving a 0.5 mL sample of the PLO emulsion in 20 mL of tetrahydrofuran and reading the dissolved sample.
Standard Solutions: Standard preparations of 80% to 110% of ketoprofen were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 1.0 g/10 mL

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A 250 mg sample was removed from the top of the stick, dissolved in 20 mL of tetrahydrofuran, and when dissolution was complete, the mixture was hand-shaken to mix well. The samples were left to stand overnight. One (1) mL of the supernatant was further diluted with 10 mL of tetrahydrofuran and was read.
Standard Solutions: Standard preparations of 2.0% to 3.0% of hydrocortisone medication sticks were prepared in the same manner as the student preparations, and were used as a linear standard curve to determine the student preparation percentage.
Expected Concentration of API: 2.5%

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A tablet was selected at random from the compounded tablets and dissolved in 20mL of distilled water and read.
Standard Solutions: Standard preparations of 10-15 mg/tablet of metoprolol were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 12.5 mg per tablet

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A tablet was selected at random and dissolved into 20 mL of 75% methanol and 25% distilled water. Samples were sonicated for approximately 20 minutes until dissolved.
Standard Solutions: Standard preparations of 5-15 mg/tablet of furosemide were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 10 mg per tablet

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A troche was selected at random and dissolved in 20 mL of pH 10 buffer. The samples were left to stand overnight and read.

• pH 10 buffer:
  • Solution A:
    • 12.37 g boric acid
    • 100 mL 1.0 N sodium hydroxide
    • qs 1 L distilled water
  • Solution B:
    • 0.1 N sodium hydroxide
  • Combine 59.6 mL of Solution A with 40.4 mL of solution B to get the pH 10 buffer used for sample preparation

Standard Solutions: Standard preparations of 50-150 mg/troche of tartrazine were prepared in the same manner as the student preparations, and were used as a linear standard curve to determine the student preparation amount. Make sure the standards are left to stand overnight in order to get a linear curve.
Expected Concentration of API: 100 mg tartrazine per troche

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A troche was selected at random and dissolved in 20 mL of pH 10 buffer. A second dilution was prepared by adding 2 mL of the first dilution into 20 mL of pH 10 buffer and was then read.

• pH 10 buffer:
  • Solution A:
    • 12.37 g boric acid
    • 100 mL 1.0 N sodium hydroxide
    • qs 1 L distilled water
  • Solution B:
    • 0.1 N sodium hydroxide
  • Combine 59.6 mL of Solution A with 40.4 mL of Solution B to get the pH 10 buffer used for sample preparation.

Standard Solutions: Standard preparations of 8.3-20.8 mg/troche of methanol were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount. Make sure the standards are left to stand overnight in order to get a linear curve.
Expected Concentration of API: 15 mg of phenol per troche

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A 0.5 g sample was dissolved in 20 mL of ethanol and left to stand overnight. Without shaking the first dilution, 0.5 mL of the first dilution was pipetted into 20 mL of ethanol and read. It is important to pre-wet the pipette tip before drawing up the 0.5 mL sample.
Standard Solutions: Standard preparations of 1.5% to 2.5% lidocaine gel were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 2% lidocaine

Link to Formulation Record:

Process of Analysis:

Sample Preparation: A 0.5 mL sample was diluted into 20 mL of methanol. A second dilution was made by adding 0.1 mL of the first dilution into 9 mL of methanol. The second dilution was left to stand overnight then read.
Standard Solutions: Standard preparations of 0% to 23.3% salicylic acid in flexible collodion were prepared in the same manner as the student preparations and were used as a linear standard curve to determine the student preparation amount.
Expected Concentration of API: 16.7% salicylic acid