Powders & Granules: Labs

Laboratory Procedure

A. Preparation of Divided Powder

1. Compound and dispense the following prescription.

                 The University of North Carolina     Rx# 61601

                         School of Pharmacy

                         Chapel Hill, NC

Phone:  555-1234                                 DEA# PS0079411



Name:  Pete E. Arick, Jr. (age 11 months)       Date:   Today  

Address:  300 Graham St., Chapel Hill, NC 27599 Phone:  911-1234



  R_x

               Salicylic Acid   30 mg

               Lactose q.s.    250 mg

               M.Ft. chart DTD #10

               Sig: 1 Chart in juice q 4h prn T > 99.5



  No Refills



  Angela Murcee, M.D.                                        

  Dispense as written             Product selection permitted

Procedure:

Using geometric dilution, triturate the components in a mortar and prepare 10 powder charts (making calculations for 12 charts). Dispense 6 charts and save 4 for subsequent testing.

View a video demonstration on geometric dilution

2. Evaluation of Divided Powders

Empty the contents of each of the 4 reserved charts into 4 Erlenmeyer flasks. Add 100 ml of 0.01 N NaOH to each flask and stir to effect dissolution. Determine the absorbance of each sample at 325 nm on a spectrophotometer. Prepare a blank of 0.01 N NaOH.

Using a set of reference standards of known concentration, prepare a Beer’s Law plot (absorbance vs. concentration). Using this Beer’s Law data, calculate the amount of drug in each chart, and calculate the mean, standard deviation and relative standard deviation of your results.

B. Preparation of Effervescent Granulation

1. Compound and dispense the following prescription.

                 The University of North Carolina        Rx# 61602

                         School of Pharmacy

                         Chapel Hill, NC

Phone:  555-1234                                      DEA# PS0079411



Name:  Ben D. Rools                                 Date:   Today  

Address:  112 N. Church St., Chapel Hill, NC 27599  Phone:  654-3211



  R_x

                 Aspirin                 gr. 80

                 Sodium Bicarbonate      gr. 220

                 Tartaric Acid           gr. 120

                 Citric Acid Monohydrate gr. 80

                 M.Ft. granules

                 Sig: 1 tsp q 6h prn pain

  2 Refills



  Mel Praktisute, M.D.                                     

  Dispense as written          Product selection permitted

Procedure:

Mix all components using geometric dilution. Pass the powder mass through a 40-60 mesh sieve. Package in dry, airtight container.

C. Preparation of Dusting Powder

1. Compound and dispense the following prescription.

                 The University of North Carolina        Rx# 61603

                         School of Pharmacy

                         Chapel Hill, NC

Phone:  555-1234                                        DEA# PS0079411



Name:  Wendy Wenbloes                                 Date:   Today  

Address:  118 Summerbreeze Dr, Chapel Hill, NC 27599  Phone:  667-1110



  R_x

               Calamine              15%

               Magnesium Stearate     1%

               Talc q.s.             50 g

               M.Ft. pulv

               Sig: Dust affected area t.i.d.



  No Refills



  Lee Keybote, M.D.                                          

  Dispense as written             Product selection permitted

Procedure:

Using geometric dilution, triturate the components in a mortar. Pass the powder mass through a 100-200 mesh sieve.

View a video demonstration on how to sieve a powder

Transfer carefully into a sifter top container. (If sifter top container unavailable, use wide mouth jar and provide patient with instructions for administering the powder). Avoid inhaling the dust.

I. Prelab data

Preparation of Divided Powder (Rx #61601)

Compounding Formula for Twelve Charts:

Salicylic Acid	_____________ mg
Lactose	_____________ mg
Total Weight of 12 Charts	_____________ mg
Weight of Each Chart	_____________ mg

Preparation of Effervescent Granulation (Rx #61602)

Compounding Formula:

Aspirin	______________ g
Sodium Bicarbonate	______________ g
Tartaric Acid	______________ g
Citric Acid Monohydrate	______________ g
Total Weight of Granulation	______________ g

Preparation of Dusting Powder (Rx# 61603)

Compounding Formula:

Calamine	______________ g
Magnesium Stearate	______________ g
Talc	______________ g
Total Weight of Powder	______________ g

II. Experimental Data and Results

A. Divided Powders

- Inspection of Final Product ______________________
- Evaluation of Divided Powders
  - Prepare a Beer’s Law plot and show the slope, intercept, and R² values. Attach graph.

SA Standard	Absorbance @ 325 nm	Regression Analysis
0.4 mg/ml	_______________	intercept = _______
0.3 mg/ml	_______________
0.2 mg/ml	_______________	slope = __________
0.1 mg/ml	_______________
0.0 mg/ml	_______________	R² = ____________

b. Complete the following table:

Sample	Absorbance @ 325 nm	Concentration	Amount of Drug Per Chart
1
2
3
4

Average ____________________

Standard Deviation ____________

B. Granulation

C. Bulk Powder

III. Discussion

1. Discuss the content uniformity of the powder charts you prepared. Is it within acceptable limits?

2. How would you instruct a patient to take Rx #61602?

3. If sodium carbonate is to be used instead of sodium bicarbonate in the preparation of effervescent granules, what would be the amount needed to neutralize three (3) gm of citric and tartaric acids in a ratio of 1:2 respectively?

4. What is the role or purpose of each ingredient in the dusting powder you prepared?

Calamine –

Talc –

Magnesium stearate –